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KMID : 0368420020450010022
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Journal of Plant Biology
2002 Volume.45 No. 1 p.22 ~ p.27
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Callus Induction and Plantlet Regeneration from Mature Cotyledonary Segments of Groundnut(Arachis hypogaea L.)
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Palanivel, S.
Parvathi, S./Jayabalan, N.
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Abstract
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We evaluated the efficiency of callus induction and plantlet regeneration from mature cotyledonary segments of groundnut cultivars VRI-2 and VRI-3. Callus cultures were induced from mature tissues using NAA and IAA in combination with KIN or BAP. Maximum induction was recorded with 3.0㎎/L IAA and 1.0㎎/L BAP. However, green, compact, and nodular calli were obtained in 2.5㎎/L of IAA or NAA combined with 1.0㎎/L of either BAP or KIN. Fresh and dry weights were highly influenced by auxin concentration. Compact and nodular call! were then transferred to shoot induction media. The highest mean number of shoots was observed in 3.0㎎/L BAP plus 0.5㎎/L IAA. Finally, the resulting plantlets were rooted with IBA and NAA.
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